Hotspot

ndhF-rpl32

SSC · 118994-119868 · 875 bp

Back to Bistorta

Pi 0.0341
MarkerSeek score 66.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
875
variable_sites
68
pi
0.0341
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.93
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCGCTCCGATTAACAAAGGA ACATCAAAAATCGGGGAGGG 50.0 50.0 58.0 57.9 1569-1586 bp 0.222222 48.6
ndhF-rpl32_p2 TCCCGCTCCGATTAACAAAG ACATCAAAAATCGGGGAGGG 50.0 50.0 58.0 57.9 1571-1588 bp 0.222222 48.6
ndhF-rpl32_p3 CCGCTCCGATTAACAAAGGA GCGGTTGATGGGGAAAGTAA 50.0 50.0 58.0 58.2 1437-1454 bp 0.222222 48.5
ndhF-rpl32_p4 TCCCGCTCCGATTAACAAAG GCGGTTGATGGGGAAAGTAA 50.0 50.0 58.0 58.2 1439-1456 bp 0.222222 48.5
ndhF-rpl32_p5 TACATGAAGGGGGAATTGCG ACATCAAAAATCGGGGAGGG 50.0 50.0 57.9 57.9 2240-2257 bp 0.222222 48.0