Hotspot

rpl33-rps18

LSC · 69263-69769 · 507 bp

Back to Bistorta

Pi 0.0488

MarkerSeek score 67.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 507
variable_sites: 50
pi: 0.0488
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CAAAAGTCTCATACGCGGGA	GCAATTCGCGGAACAGAAAA	50.0	45.0	58.0	58.0	1730-1734 bp	0.222222	49.3
rpl33-rps18_p2	AAAAGTCTCATACGCGGGAC	GCAATTCGCGGAACAGAAAA	50.0	45.0	58.0	58.0	1729-1733 bp	0.222222	49.3
rpl33-rps18_p3	CACACCGAGTCGATTGGAAT	GCAATTCGCGGAACAGAAAA	50.0	45.0	58.0	58.0	1327-1331 bp	0.222222	49.3
rpl33-rps18_p4	CAAAAGTCTCATACGCGGGA	GCAGTTCTAACGGTCGACTT	50.0	50.0	58.0	57.9	1258-1270 bp	0.222222	49.3
rpl33-rps18_p5	GCTTCGCGTCGAAAAGAAAA	GCAATTCGCGGAACAGAAAA	45.0	45.0	58.0	58.0	1510-1514 bp	0.222222	49.2