Hotspot

trnF-GAA-ndhJ

LSC · 50039-50757 · 719 bp

Back to Bistorta

Pi 0.0253

MarkerSeek score 68.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 49
pi: 0.0253
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TCCACGCCTGAAACGTATTT	50.0	45.0	58.1	57.8	846-869 bp	1.0	84.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	824-847 bp	1.0	83.5
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CATCCACGCCTGAAACGTAT	50.0	50.0	58.1	58.3	848-871 bp	1.0	83.3
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATT	50.0	45.0	58.1	57.6	847-870 bp	1.0	82.9
trnF-GAA-ndhJ_p5	TCGGGATAGCTCAGTTGGTA	TCCACGCCTGAAACGTATTT	50.0	45.0	57.6	57.8	884-907 bp	1.0	81.3