Hotspot

trnS-GCU-trnG-UCC

LSC · 7870-8675 · 806 bp

Back to Bistorta

Pi 0.0515

MarkerSeek score 77.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 806
variable_sites: 86
pi: 0.0515
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	TCCACTCAGCCATCTCTCC	GGAATCAAATCGAAGGATCCCT	57.9	45.5	58.1	57.9	771-894 bp	1.0	66.5
trnS-GCU-trnG-UCC_p2	GTCCACTCAGCCATCTCTCC	GGAATCAAATCGAAGGATCCCT	60.0	45.5	59.5	57.9	772-895 bp	1.0	63.0
trnS-GCU-trnG-UCC_p3	TCCACTCAGCCATCTCTCC	GGAATCAAATCGAAGGATCCC	57.9	47.6	58.1	56.5	771-894 bp	1.0	62.9
trnS-GCU-trnG-UCC_p4	GTCCACTCAGCCATCTCTCC	GGAATCAAATCGAAGGATCCC	60.0	47.6	59.5	56.5	772-895 bp	1.0	59.3
trnS-GCU-trnG-UCC_p5	TCCACTCAGCCATCTCTCC	TCGAAGGATCCCTTAACTCT	57.9	45.0	58.1	55.0	762-885 bp	1.0	58.9