Hotspot

rps16-trnQ-UUG

LSC · 6029-6558 · 530 bp

Back to Bistorta

Pi 0.0509
MarkerSeek score 79.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
530
variable_sites
70
pi
0.0509
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.92
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CGTTGCTTTCTACCACATCG TTCGAAATCTAAGTGGGGCG 50.0 50.0 57.2 58.0 626-660 bp 1.0 85.5
rps16-trnQ-UUG_p2 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 565-599 bp 1.0 85.1
rps16-trnQ-UUG_p3 GCACGTTGCTTTCTACCACA TTCGAAATCTAAGTGGGGCG 50.0 50.0 59.1 58.0 629-663 bp 1.0 83.4
rps16-trnQ-UUG_p4 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 50.0 55.0 59.1 58.1 568-602 bp 1.0 82.9
rps16-trnQ-UUG_p5 CGTTGCTTTCTACCACATCG GAAATCTAAGTGGGGCGTGG 50.0 55.0 57.2 58.6 623-657 bp 1.0 80.7