Hotspot

psbA-trnK-UUU

LSC · 1327-1553 · 227 bp

Back to Bistorta

Pi 0.0481

MarkerSeek score 68.10

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 227
variable_sites: 24
pi: 0.0481
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	CGTCTCTCTAAAATTGCAGTCA	ACTCCATCCGACTAGTTCCG	40.9	55.0	56.5	58.6	292-315 bp	1.0	53.7
psbA-trnK-UUU_p2	CGTCTCTCTAAAATTGCAGTCA	ATCCGACTAGTTCCGGGTTC	40.9	55.0	56.5	58.9	287-310 bp	1.0	51.6
psbA-trnK-UUU_p3	CGTCTCTCTAAAATTGCAGTCAT	ACTCCATCCGACTAGTTCCG	39.1	55.0	57.0	58.6	292-315 bp	1.0	49.5
psbA-trnK-UUU_p4	CGTCTCTCTAAAATTGCAGTCA	CATCCGACTAGTTCCGGGTT	40.9	55.0	56.5	59.2	288-311 bp	1.0	49.3
psbA-trnK-UUU_p5	CGTCTCTCTAAAATTGCAGTCA	TCCATCCGACTAGTTCCGG	40.9	57.9	56.5	58.2	290-313 bp	1.0	49.1