Hotspot

psbA

LSC · 327-1388 · 1062 bp

Back to Biscutella

Pi 0.00847

MarkerSeek score 50.20

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 17
pi: 0.00847
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	CTGCCTTAATCCACTTGGCT	ATTTGTGTGCTTGGGAGTCC	50.0	50.0	57.9	58.4	1396-1408 bp	0.5	59.3
psbA_p2	ACTGCCTTAATCCACTTGGC	ATTTGTGTGCTTGGGAGTCC	50.0	50.0	58.2	58.4	1397-1409 bp	0.5	59.1
psbA_p3	CTGCCTTAATCCACTTGGCT	AAAATGCAAGCACGGTTTGG	50.0	45.0	57.9	58.4	1727-1733 bp	0.5	58.9
psbA_p4	ACTGCCTTAATCCACTTGGC	AAAATGCAAGCACGGTTTGG	50.0	45.0	58.2	58.4	1728-1734 bp	0.5	58.6
psbA_p5	CTGCCTTAATCCACTTGGCT	TTACGGGGGAAGACCAAGTT	50.0	50.0	57.9	58.6	2076-2082 bp	0.5	58.4