Hotspot

rpl22-rps19

LSC · 83316-83375 · 60 bp

Back to Biscutella

Pi 0.0472

MarkerSeek score 73.10

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 5
pi: 0.0472
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	ACGTGCAATTCCCTCCATAC	CCGGATCGAAATGTTGGCTA	50.0	50.0	58.0	58.1	1254-1260 bp	1.0	81.1
rpl22-rps19_p2	TCATTGCTGCATCCAGGTTT	CCGGATCGAAATGTTGGCTA	45.0	50.0	58.1	58.1	1023-1029 bp	1.0	81.1
rpl22-rps19_p3	TTCATTGCTGCATCCAGGTT	CCGGATCGAAATGTTGGCTA	45.0	50.0	58.1	58.1	1024-1030 bp	1.0	81.1
rpl22-rps19_p4	TTTCATTGCTGCATCCAGGT	CCGGATCGAAATGTTGGCTA	45.0	50.0	58.1	58.1	1025-1031 bp	1.0	81.1
rpl22-rps19_p5	ACGTGCAATTCCCTCCATAC	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.0	58.1	1270-1276 bp	1.0	80.8