Hotspot

rpl36-rps8

LSC · 78884-79365 · 482 bp

Back to Biscutella

Pi 0.043

MarkerSeek score 78.70

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 482
variable_sites: 35
pi: 0.043
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TTTTACGAACGGAAGCCCTT	TCGGCGGAGAAATTTTGTGT	45.0	45.0	57.7	58.4	519-542 bp	1.0	83.5
rpl36-rps8_p2	TTTTACGAACGGAAGCCCTT	CGGCGGAGAAATTTTGTGTT	45.0	45.0	57.7	57.6	518-541 bp	1.0	83.4
rpl36-rps8_p3	TTTTACGAACGGAAGCCCTT	ATTCCACGGATTTTAGGCGG	45.0	50.0	57.7	58.3	607-630 bp	1.0	82.9
rpl36-rps8_p4	TTTTACGAACGGAAGCCCTT	CCACGGATTTTAGGCGGAAT	45.0	50.0	57.7	58.3	604-627 bp	1.0	82.9
rpl36-rps8_p5	ACGAACGGAAGCCCTTATTT	CCACGGATTTTAGGCGGAAT	45.0	50.0	57.5	58.3	600-623 bp	1.0	81.2