Hotspot

petB-petD

LSC · 75585-75781 · 197 bp

Back to Biscutella

Pi 0.0415

MarkerSeek score 73.10

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 13
pi: 0.0415
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	GCAAGGTATTTCTGGTCCCT	CGGCCCGATCAATAGTTCAA	50.0	50.0	57.2	58.1	257 bp	1.0	81.2
petB-petD_p2	AGCAAGGTATTTCTGGTCCC	CGGCCCGATCAATAGTTCAA	50.0	50.0	57.2	58.1	258 bp	1.0	81.1
petB-petD_p3	AGCAAGGTATTTCTGGTCCC	GGCTTACACAGTGGTTGGAA	50.0	50.0	57.2	58.0	333 bp	1.0	79.6
petB-petD_p4	GCAAGGTATTTCTGGTCCCT	GGCTTACACAGTGGTTGGAA	50.0	50.0	57.2	58.0	332 bp	1.0	79.6
petB-petD_p5	GCAAGGTATTTCTGGTCCCTT	GGCTTACACAGTGGTTGGAA	47.6	50.0	57.9	58.0	332 bp	1.0	77.4