Hotspot

trnW-trnP

LSC · 65464-65661 · 198 bp

Back to Biscutella

Pi 0.0532

MarkerSeek score 81.70

Primer available yes

Label trnW-trnP

Feature Metadata

label_name: trnW-trnP
feature_type: igs
region: LSC
length_bp: 198
variable_sites: 19
pi: 0.0532
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-trnP_p1	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	260-265 bp	1.0	86.8
trnW-trnP_p2	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	262-267 bp	1.0	86.7
trnW-trnP_p3	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	263-268 bp	1.0	86.7
trnW-trnP_p4	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	273-278 bp	1.0	86.5
trnW-trnP_p5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	274-279 bp	1.0	86.4