Hotspot

ndhC-trnV

LSC · 50045-50797 · 753 bp

Back to Biscutella

Pi 0.0413

MarkerSeek score 75.00

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 753
variable_sites: 55
pi: 0.0413
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	CTAGAAAGTTTCTCCGGCCC	AAATGCTCGGCAGAATGAGT	55.0	45.0	58.0	57.9	1289-1307 bp	0.75	74.6
ndhC-trnV_p2	CATTGCCCACGGATACAGAA	AAATGCTCGGCAGAATGAGT	50.0	45.0	58.0	57.9	1413-1431 bp	0.75	74.2
ndhC-trnV_p3	AACTCATTGCCCACGGATAC	AAATGCTCGGCAGAATGAGT	50.0	45.0	58.0	57.9	1417-1435 bp	0.75	74.1
ndhC-trnV_p4	GGCTGGAAAGTCTTGACCAA	AAATGCTCGGCAGAATGAGT	50.0	45.0	58.0	57.9	1684-1706 bp	0.75	74.0
ndhC-trnV_p5	GGATACCCCCAGTACATCGA	AAATGCTCGGCAGAATGAGT	55.0	45.0	58.0	57.9	1443-1455 bp	0.5	62.8