Hotspot

trnR-atpA

LSC · 9479-9791 · 313 bp

Back to Biscutella

Pi 0.0598

MarkerSeek score 78.20

Primer available yes

Label trnR-atpA

Feature Metadata

label_name: trnR-atpA
feature_type: igs
region: LSC
length_bp: 313
variable_sites: 30
pi: 0.0598
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-atpA_p1	GCGAAAAGCGTCCATTGTC	GAACGTTTTCTACTTCAAGAGA	52.6	36.4	58.0	54.1	397-422 bp	1.0	49.9
trnR-atpA_p2	GCGAAAAGCGTCCATTGTC	GAACGTTTTCTACTTCAAGAGAA	52.6	34.8	58.0	54.9	397-422 bp	1.0	49.9
trnR-atpA_p3	GCGAAAAGCGTCCATTGTC	AACGTTTTCTACTTCAAGAGA	52.6	33.3	58.0	52.8	396-421 bp	1.0	49.9
trnR-atpA_p4	GCGAAAAGCGTCCATTGTC	ACGTTTTCTACTTCAAGAGAA	52.6	33.3	58.0	52.8	395-420 bp	1.0	49.9
trnR-atpA_p5	GCGAAAAGCGTCCATTGTC	ACGTTTTCTACTTCAAGAGAAA	52.6	31.8	58.0	53.7	395-420 bp	1.0	49.9