Hotspot

trnS-trnT

LSC · 7835-8451 · 617 bp

Back to Biscutella

Pi 0.0404

MarkerSeek score 75.00

Primer available yes

Label trnS-trnT

Feature Metadata

label_name: trnS-trnT
feature_type: igs
region: LSC
length_bp: 617
variable_sites: 43
pi: 0.0404
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnT_p1	CCAACGCTTTAGTCCACTCA	ACGAATCACACTTTTACCACT	50.0	38.1	57.8	55.5	682-785 bp	1.0	56.3
trnS-trnT_p2	AACGCTTTAGTCCACTCAGC	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	680-783 bp	1.0	56.0
trnS-trnT_p3	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	50.0	38.1	57.6	55.5	677-780 bp	1.0	54.4
trnS-trnT_p4	TCCAACGCTTTAGTCCACTC	ACGAATCACACTTTTACCACT	50.0	38.1	57.5	55.5	683-786 bp	1.0	54.0
trnS-trnT_p5	AGTCCACTCAGCCATCTCTC	ACGAATCACACTTTTACCACT	55.0	38.1	58.5	55.5	672-775 bp	1.0	53.6