Hotspot

trnK-trnQ

LSC · 4285-6502 · 2218 bp

Back to Biscutella

Pi 0.0356

MarkerSeek score 74.80

Primer available yes

Label trnK-trnQ

Feature Metadata

label_name: trnK-trnQ
feature_type: igs
region: LSC
length_bp: 2218
variable_sites: 137
pi: 0.0356
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-trnQ_p1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2255-2351 bp	1.0	86.8
trnK-trnQ_p2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2254-2350 bp	1.0	86.8
trnK-trnQ_p3	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	2241-2337 bp	1.0	84.5
trnK-trnQ_p4	AGTCGCACTTAAAAGCCGAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.6	58.1	2266-2362 bp	1.0	83.6
trnK-trnQ_p5	TCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.3	58.1	2264-2360 bp	1.0	82.3