Hotspot

rpl32-ndhF

SSC · 123644-124706 · 1063 bp

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Pi 0.0117
MarkerSeek score 57.90
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
SSC
length_bp
1063
variable_sites
79
pi
0.0117
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.91
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 TTTAACGACGCCCCATATCC CCGTCGGGAAAAGAAGAAGT 50.0 50.0 57.8 57.8 1171-1246 bp 1.0 86.3
rpl32-ndhF_p2 CAAAGCTTTTAACGACGCCC CCGTCGGGAAAAGAAGAAGT 50.0 50.0 58.3 57.8 1178-1253 bp 1.0 85.7
rpl32-ndhF_p3 CGCCCCATATCCTTTCCTTT CCGTCGGGAAAAGAAGAAGT 50.0 50.0 57.6 57.8 1163-1238 bp 1.0 85.3
rpl32-ndhF_p4 ACAAAGCTTTTAACGACGCC CCGTCGGGAAAAGAAGAAGT 45.0 50.0 57.6 57.8 1179-1254 bp 1.0 85.0
rpl32-ndhF_p5 TAACGACGCCCCATATCCTT CCGTCGGGAAAAGAAGAAGT 50.0 50.0 58.6 57.8 1169-1244 bp 1.0 83.6