Hotspot

trnL-UAG-rpl32

SSC · 122623-123478 · 856 bp

Back to Bidens

Pi 0.0131
MarkerSeek score 75.80
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
856
variable_sites
88
pi
0.0131
unique_haplotype_count
24
primer_available
yes
alignment_reliability
0.96
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC GGATATGGGGCGTCGTTAAA 50.0 50.0 58.0 57.8 932-985 bp 1.0 89.4
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA GGATATGGGGCGTCGTTAAA 50.0 50.0 58.0 57.8 933-986 bp 1.0 89.3
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC GGGCGTCGTTAAAAGCTTTG 50.0 50.0 58.0 58.3 925-978 bp 1.0 88.8
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA GGGCGTCGTTAAAAGCTTTG 50.0 50.0 58.0 58.3 926-979 bp 1.0 88.8
trnL-UAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA GGATATGGGGCGTCGTTAAA 50.0 50.0 57.9 57.8 942-995 bp 1.0 88.4