Hotspot

trnR-ACG-trnN-GUU

IRb · 107137-107601 · 465 bp

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Pi 0.00233
MarkerSeek score 44.20
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
465
variable_sites
11
pi
0.00233
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.8 58.0 605-606 bp 1.0 79.8
trnR-ACG-trnN-GUU_p2 TTTGAGGGTACTTCCGGGAA CCGTTGTTCCTCAGTAGCTC 50.0 55.0 58.3 58.0 659-660 bp 1.0 79.3
trnR-ACG-trnN-GUU_p3 TTTTGAGGGTACTTCCGGGA CCGTTGTTCCTCAGTAGCTC 50.0 55.0 58.3 58.0 660-661 bp 1.0 79.3
trnR-ACG-trnN-GUU_p4 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.8 58.2 556-557 bp 1.0 78.7
trnR-ACG-trnN-GUU_p5 CAGAGGATTAGAGCACGTGG GGTTGAGAACGGGAATGGAA 55.0 50.0 57.8 57.8 644-645 bp 1.0 78.2