Hotspot

trnT-GGU-psbD

LSC · 31051-32225 · 1175 bp

Back to Bidens

Pi 0.00903
MarkerSeek score 50.20
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1175
variable_sites
54
pi
0.00903
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.96
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTAATCCCATGGTAACGCGT GAAACGGTCCCTACGTAACC 50.0 55.0 58.1 58.0 1276-1309 bp 1.0 87.1
trnT-GGU-psbD_p2 GTAATCCCATGGTAACGCGT GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1294-1327 bp 1.0 86.2
trnT-GGU-psbD_p3 AGTAATCCCATGGTAACGCG GAAACGGTCCCTACGTAACC 50.0 55.0 57.8 58.0 1277-1310 bp 1.0 85.7
trnT-GGU-psbD_p4 ATCCCATGGTAACGCGTAAG GAAACGGTCCCTACGTAACC 50.0 55.0 57.8 58.0 1273-1306 bp 1.0 85.7
trnT-GGU-psbD_p5 GTAATCCCATGGTAACGCGT ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1284-1317 bp 0.962963 82.2