Hotspot

psbM-trnD-GUC

LSC · 10837-11484 · 648 bp

Back to Bidens

Pi 0.00453
MarkerSeek score 57.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
648
variable_sites
28
pi
0.00453
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TTGACTGGCTGTTTTTACGT TTCAATAGGCAAGAGCACCG 40.0 50.0 55.8 58.3 795-821 bp 1.0 65.2
psbM-trnD-GUC_p2 TTTGACTGGCTGTTTTTACGT TTCAATAGGCAAGAGCACCG 38.1 50.0 56.6 58.3 796-822 bp 1.0 63.3
psbM-trnD-GUC_p3 TTTTGACTGGCTGTTTTTACGT TTCAATAGGCAAGAGCACCG 36.4 50.0 57.3 58.3 797-823 bp 1.0 61.0
psbM-trnD-GUC_p4 TGACTGGCTGTTTTTACGTA TTCAATAGGCAAGAGCACCG 40.0 50.0 55.0 58.3 794-820 bp 1.0 58.2
psbM-trnD-GUC_p5 TTGACTGGCTGTTTTTACGT GTTCAATAGGCAAGAGCACC 40.0 50.0 55.8 56.8 796-822 bp 1.0 57.5