Hotspot

rps16-trnQ-UUG

LSC · 6329-7349 · 1021 bp

Back to Bidens

Pi 0.00595
MarkerSeek score 54.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1021
variable_sites
56
pi
0.00595
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.95
markerseek_score
54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1061-1112 bp 1.0 86.9
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1044-1095 bp 1.0 86.5
rps16-trnQ-UUG_p3 CAACGGATCGTGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1062-1113 bp 1.0 85.9
rps16-trnQ-UUG_p4 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1059-1110 bp 1.0 84.6
rps16-trnQ-UUG_p5 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1058-1109 bp 1.0 84.6