Hotspot

trnK-UUU-rps16

LSC · 4346-5165 · 820 bp

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Pi 0.0037
MarkerSeek score 58.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
820
variable_sites
28
pi
0.0037
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.96
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 683-1017 bp 1.0 83.5
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 622-956 bp 1.0 82.9
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 621-955 bp 1.0 82.9
trnK-UUU-rps16_p4 TCCTAGCCGCACTTAAAAGC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.3 58.3 637-971 bp 1.0 82.1
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.3 58.3 631-965 bp 1.0 82.0