Hotspot

ndhG-ndhI

SSC · 121122-121459 · 338 bp

Back to Biancaea

Pi 0.127

MarkerSeek score 83.60

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 338
variable_sites: 42
pi: 0.127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ACAAGACCTGATCCCAGAAAG	AATCAAATTGCTTTGGGCCG	47.6	45.0	57.3	57.9	472-549 bp	1.0	73.4
ndhG-ndhI_p2	ACAAGACCTGATCCCAGAAAG	TTTGGGCCGTTTACCAATGT	47.6	45.0	57.3	58.3	461-538 bp	1.0	72.0
ndhG-ndhI_p3	ACAAGACCTGATCCCAGAAA	AATCAAATTGCTTTGGGCCG	45.0	45.0	56.0	57.9	472-549 bp	1.0	71.4
ndhG-ndhI_p4	ACAAGACCTGATCCCAGAAAG	CTTTGGGCCGTTTACCAATG	47.6	50.0	57.3	57.6	462-539 bp	1.0	71.4
ndhG-ndhI_p5	CAAGACCTGATCCCAGAAAGA	AATCAAATTGCTTTGGGCCG	47.6	45.0	57.0	57.9	471-548 bp	1.0	71.2