Hotspot

ccsA-ndhD

SSC · 117605-117915 · 311 bp

Back to Biancaea

Pi 0.0337

MarkerSeek score 64.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 311
variable_sites: 10
pi: 0.0337
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGGACTACATAGTTATGGTTCA	CAGTTGACAAGGTCGAAGCT	36.4	50.0	53.7	58.1	389-407 bp	1.0	44.8
ccsA-ndhD_p2	AGGACTACATAGTTATGGTTCA	TCAGTTGACAAGGTCGAAGC	36.4	50.0	53.7	58.1	390-408 bp	1.0	44.8
ccsA-ndhD_p3	AGGACTACATAGTTATGGTTCAT	CAGTTGACAAGGTCGAAGCT	34.8	50.0	54.3	58.1	389-407 bp	1.0	44.8
ccsA-ndhD_p4	AGGACTACATAGTTATGGTTCAT	TCAGTTGACAAGGTCGAAGC	34.8	50.0	54.3	58.1	390-408 bp	1.0	44.8
ccsA-ndhD_p5	GGACTACATAGTTATGGTTCA	CAGTTGACAAGGTCGAAGCT	38.1	50.0	52.1	58.1	388-406 bp	1.0	44.8