Hotspot

ndhF-rpl32

SSC · 115138-115898 · 761 bp

Back to Biancaea

Pi 0.0908

MarkerSeek score 80.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 761
variable_sites: 68
pi: 0.0908
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TAGGGCGTGTGAACCAAAAA	TCAATGCTGCCCAATATCCC	45.0	50.0	57.9	58.0	1357-1447 bp	1.0	84.4
ndhF-rpl32_p2	TAGGGCGTGTGAACCAAAAA	CAATGCTGCCCAATATCCCT	45.0	50.0	57.9	58.0	1356-1446 bp	1.0	84.3
ndhF-rpl32_p3	TAGGGCGTGTGAACCAAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	1851-1946 bp	1.0	83.8
ndhF-rpl32_p4	TAGGGCGTGTGAACCAAAAA	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	1852-1947 bp	1.0	83.8
ndhF-rpl32_p5	AGCAACTGAACCGCCAAATA	TCAATGCTGCCCAATATCCC	45.0	50.0	58.1	58.0	1566-1656 bp	1.0	83.4