Hotspot

rps8-rpl14

LSC · 83114-83347 · 234 bp

Back to Biancaea

Pi 0.0565

MarkerSeek score 69.70

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 13
pi: 0.0565
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GTCGAATCCTTCCGGAAACA	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.9	57.9	917-1114 bp	1.0	80.6
rps8-rpl14_p2	GTCGAATCCTTCCGGAAACA	TATGGGGTCGGGAAAAGGAT	50.0	50.0	57.9	58.1	1569-1761 bp	1.0	80.5
rps8-rpl14_p3	TGTCGAATCCTTCCGGAAAC	TATGGGGTCGGGAAAAGGAT	50.0	50.0	57.9	58.1	1570-1762 bp	1.0	80.5
rps8-rpl14_p4	GTCGAATCCTTCCGGAAACA	TGATAATGCTGCGGTTGTCA	50.0	45.0	57.9	57.9	1020-1217 bp	1.0	80.4
rps8-rpl14_p5	TGTCGAATCCTTCCGGAAAC	TGATAATGCTGCGGTTGTCA	50.0	45.0	57.9	57.9	1021-1218 bp	1.0	80.4