Hotspot

ndhC-trnV-UAC

LSC · 53543-54251 · 709 bp

Back to Biancaea

Pi 0.0341
MarkerSeek score 66.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
709
variable_sites
24
pi
0.0341
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCTTTGCTAATCGGGGCTAA GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.7 58.7 840-1023 bp 1.0 74.3
ndhC-trnV-UAC_p2 CCTTTGCTAATCGGGGCTAA TTCGAGTCCGTATAGCCCTA 50.0 50.0 57.7 57.1 839-1022 bp 1.0 72.3
ndhC-trnV-UAC_p3 CTCTGGCCCTTTGCTAATCG GTTCGAGTCCGTATAGCCCT 55.0 55.0 58.7 58.7 847-1030 bp 1.0 71.4
ndhC-trnV-UAC_p4 TCTCTGGCCCTTTGCTAATC GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.3 58.7 848-1031 bp 1.0 71.3
ndhC-trnV-UAC_p5 CTCTGGCCCTTTGCTAATCG TTCGAGTCCGTATAGCCCTA 55.0 50.0 58.7 57.1 846-1029 bp 1.0 69.4