Hotspot

trnF-GAA-ndhJ

LSC · 51127-51861 · 735 bp

Back to Biancaea

Pi 0.0138

MarkerSeek score 65.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 10
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTGC	50.0	50.0	58.1	57.9	832-839 bp	1.0	80.8
trnF-GAA-ndhJ_p2	GAGGATGATGCGTCACGAAT	TTGGATAGGATGGCCTTTGC	50.0	50.0	58.1	57.9	892-899 bp	1.0	80.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	843-850 bp	1.0	78.6
trnF-GAA-ndhJ_p4	GAGGATGATGCGTCACGAAT	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	903-910 bp	1.0	77.8
trnF-GAA-ndhJ_p5	AGGATGATGCGTCACGAATG	TTGGATAGGATGGCCTTTGC	50.0	50.0	58.4	57.9	891-898 bp	1.0	77.7