Hotspot

psbZ-trnG-UCC

LSC · 38341-38731 · 391 bp

Back to Biancaea

Pi 0.126
MarkerSeek score 86.10
Primer available yes
Label psbZ-trnG-UCC

Feature Metadata

label_name
psbZ-trnG-UCC
feature_type
igs
region
LSC
length_bp
391
variable_sites
46
pi
0.126
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
86.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-UCC_p1 GATGGTTGGTCGAGTAACAA GCGTCTTCTCTTTGGCAAAG 45.0 50.0 55.4 57.7 538-1072 bp 1.0 63.9
psbZ-trnG-UCC_p2 GATGGTTGGTCGAGTAACAAA GCGTCTTCTCTTTGGCAAAG 42.9 50.0 56.2 57.7 538-1072 bp 1.0 62.3
psbZ-trnG-UCC_p3 TGGTTGGTCGAGTAACAAAA GCGTCTTCTCTTTGGCAAAG 40.0 50.0 55.1 57.7 536-1070 bp 1.0 61.8
psbZ-trnG-UCC_p4 TGGTTGGTCGAGTAACAAAAA GCGTCTTCTCTTTGGCAAAG 38.1 50.0 55.9 57.7 536-1070 bp 1.0 60.3
psbZ-trnG-UCC_p5 GATGGTTGGTCGAGTAACAAAA GCGTCTTCTCTTTGGCAAAG 40.9 50.0 56.9 57.7 538-1072 bp 1.0 60.0