Hotspot

atpF-atpH

LSC · 13499-14066 · 568 bp

Back to Biancaea

Pi 0.0401

MarkerSeek score 77.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 568
variable_sites: 22
pi: 0.0401
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	TGGGCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	686-694 bp	1.0	80.6
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	AGGCAGAGGGAAAAATACGG	50.0	50.0	57.9	57.6	753-761 bp	1.0	80.1
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATACGGGG	50.0	55.0	57.9	58.6	751-759 bp	1.0	78.6
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GGCAGAGGGAAAAATACGGG	50.0	55.0	57.9	58.6	752-760 bp	1.0	78.6
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CCGAGGCAGAGGGAAAAATA	50.0	50.0	57.9	57.3	756-764 bp	1.0	77.8