Hotspot

trnS-GCU-trnG-GCC

LSC · 8501-9155 · 655 bp

Back to Biancaea

Pi 0.0572

MarkerSeek score 77.50

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 655
variable_sites: 37
pi: 0.0572
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.0	58.2	724-795 bp	1.0	74.8
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCACT	50.0	42.9	58.0	58.2	723-794 bp	1.0	74.8
trnS-GCU-trnG-GCC_p3	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCAC	50.0	45.0	58.0	56.8	724-795 bp	1.0	74.7
trnS-GCU-trnG-GCC_p4	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCAC	50.0	45.0	58.0	56.8	723-794 bp	1.0	74.7
trnS-GCU-trnG-GCC_p5	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.2	58.2	778-849 bp	1.0	73.3