Hotspot

psbK-trnS-GCU

LSC · 7718-8412 · 695 bp

Back to Biancaea

Pi 0.0422

MarkerSeek score 72.90

Primer available yes

Label psbK-trnS-GCU

Feature Metadata

label_name: psbK-trnS-GCU
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 29
pi: 0.0422
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnS-GCU_p1	CTTTTGTTTGGCAAGCTGCT	TGGACTAAAGCGTCGGATTG	45.0	50.0	58.1	58.0	803-808 bp	1.0	83.1
psbK-trnS-GCU_p2	GTTTGGCAAGCTGCTGTAAG	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.9	58.0	798-803 bp	1.0	83.1
psbK-trnS-GCU_p3	CTTTTGTTTGGCAAGCTGCT	GTGGACTAAAGCGTCGGATT	45.0	50.0	58.1	58.0	804-809 bp	1.0	83.1
psbK-trnS-GCU_p4	GTTTGGCAAGCTGCTGTAAG	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.9	58.0	799-804 bp	1.0	83.1
psbK-trnS-GCU_p5	CTTTTGTTTGGCAAGCTGCT	TTTTTCGTACCGAGGGTTCG	45.0	50.0	58.1	58.2	766-771 bp	1.0	81.6