Hotspot

rpl22-rps19

LSC · 89441-89494 · 54 bp

Back to Betula

Pi 0.0422
MarkerSeek score 58.60
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
54
variable_sites
14
pi
0.0422
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TCTTCGTGCTTTGTTAGTAGACA TAAATTTCCGAGGACACGCC 39.1 50.0 57.8 58.3 151-221 bp 1.0 61.8
rpl22-rps19_p2 CGTGCTTTGTTAGTAGACATACG TAAATTTCCGAGGACACGCC 43.5 50.0 57.6 58.3 147-217 bp 1.0 60.5
rpl22-rps19_p3 CTTCGTGCTTTGTTAGTAGACA TAAATTTCCGAGGACACGCC 40.9 50.0 56.4 58.3 150-220 bp 1.0 58.7
rpl22-rps19_p4 TCGTGCTTTGTTAGTAGACA TAAATTTCCGAGGACACGCC 40.0 50.0 54.4 58.3 148-218 bp 1.0 58.5
rpl22-rps19_p5 ACCTAAAACGCATACTTCTGT TAAATTTCCGAGGACACGCC 38.1 50.0 55.3 58.3 115-185 bp 1.0 58.5