Hotspot

trnP-UGG-psaJ

LSC · 71846-72291 · 446 bp

Back to Betula

Pi 0.00388
MarkerSeek score 50.60
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
446
variable_sites
8
pi
0.00388
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ATCCCTCGTTACGGCTCATA AGTACTTACTACCGGTGCCA 50.0 50.0 58.0 57.8 570-643 bp 1.0 84.4
trnP-UGG-psaJ_p2 ATCCCTCGTTACGGCTCATA GACCTGCTAAAGACCCGAAC 50.0 55.0 58.0 58.3 595-668 bp 1.0 83.9
trnP-UGG-psaJ_p3 ATCCCTCGTTACGGCTCATA GTACTTACTACCGGTGCCAC 50.0 55.0 58.0 57.7 569-642 bp 1.0 83.9
trnP-UGG-psaJ_p4 ACCCGTGACATTTTGTACCC AGTACTTACTACCGGTGCCA 50.0 50.0 58.1 57.8 517-590 bp 1.0 83.3
trnP-UGG-psaJ_p5 CCCGTGACATTTTGTACCCA AGTACTTACTACCGGTGCCA 50.0 50.0 58.1 57.8 516-589 bp 1.0 83.3