Hotspot

trnM-CAU-atpE

LSC · 57129-57347 · 219 bp

Back to Betula

Pi 0.00781
MarkerSeek score 51.00
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
219
variable_sites
7
pi
0.00781
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 TATTGCTTTCATACGGCGGG CTCTCAGACGAGCTAGGACA 50.0 55.0 58.4 58.0 315-372 bp 1.0 80.7
trnM-CAU-atpE_p2 TATTGCTTTCATACGGCGGG CGAGCTAGGACACGAGTAGA 50.0 55.0 58.4 58.1 307-364 bp 1.0 80.6
trnM-CAU-atpE_p3 TATTGCTTTCATACGGCGGG TCTCAGACGAGCTAGGACAC 50.0 55.0 58.4 58.3 314-371 bp 1.0 78.9
trnM-CAU-atpE_p4 GTATTGCTTTCATACGGCGG CTCTCAGACGAGCTAGGACA 50.0 55.0 57.3 58.0 316-373 bp 1.0 78.7
trnM-CAU-atpE_p5 GTATTGCTTTCATACGGCGG CGAGCTAGGACACGAGTAGA 50.0 55.0 57.3 58.1 308-365 bp 1.0 78.6