Hotspot

psbZ-trnG-GCC

LSC · 40251-40751 · 501 bp

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Pi 0.0201
MarkerSeek score 66.60
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
501
variable_sites
35
pi
0.0201
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.93
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG ATTGAACCCGCATCTTCTCC 55.0 50.0 58.0 58.0 587-701 bp 1.0 87.9
psbZ-trnG-GCC_p2 CTTCTCCTGATGGTTGGTCG CCTATTACCCTGGGCGGATA 55.0 55.0 58.0 58.1 613-727 bp 1.0 87.6
psbZ-trnG-GCC_p3 TTGCTTCTCCTGATGGTTGG ATTGAACCCGCATCTTCTCC 50.0 50.0 57.8 58.0 590-704 bp 1.0 86.3
psbZ-trnG-GCC_p4 TCCTGATGGTTGGTCGAGTA ATTGAACCCGCATCTTCTCC 50.0 50.0 57.8 58.0 583-697 bp 1.0 86.2
psbZ-trnG-GCC_p5 TTGCTTCTCCTGATGGTTGG CCTATTACCCTGGGCGGATA 50.0 55.0 57.8 58.1 616-730 bp 1.0 86.1