Hotspot

trnR-UCU-atpA

LSC · 11327-11636 · 310 bp

Back to Betula

Pi 0.0103
MarkerSeek score 52.10
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
310
variable_sites
12
pi
0.0103
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC GAGGAAGCAGAAGCCCTTTT 47.6 50.0 57.3 58.1 407-432 bp 1.0 71.5
trnR-UCU-atpA_p2 GGTTCAAATCCTATTGGACGC TTCACTGAGGAAGCAGAAGC 47.6 50.0 57.3 57.8 413-438 bp 1.0 70.6
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC AGGAAGCAGAAGCCCTTTTG 47.6 50.0 57.3 58.4 406-431 bp 1.0 69.1
trnR-UCU-atpA_p4 GGTTCAAATCCTATTGGACGC GGAAGCAGAAGCCCTTTTGA 47.6 50.0 57.3 58.4 405-430 bp 1.0 69.1
trnR-UCU-atpA_p5 TGGATAGGACAGAGGTCTTCT GAGGAAGCAGAAGCCCTTTT 47.6 50.0 56.9 58.1 442-467 bp 1.0 68.2