Hotspot

trnG-UCC-trnR-UCU

LSC · 10892-11254 · 363 bp

Back to Betula

Pi 0.00922
MarkerSeek score 51.20
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
363
variable_sites
14
pi
0.00922
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.92
markerseek_score
51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 413-471 bp 1.0 69.9
trnG-UCC-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 416-474 bp 1.0 69.6
trnG-UCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 419-477 bp 1.0 69.2
trnG-UCC-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.1 57.9 422-480 bp 1.0 68.9
trnG-UCC-trnR-UCU_p5 ACCAAAGTTTCACGTTCGGT AGAAGACCTCTGTCCTATCCA 45.0 47.6 58.3 56.9 483-541 bp 1.0 67.7