Hotspot

trnK-UUU-rps16

LSC · 4453-5694 · 1242 bp

Back to Betula

Pi 0.00763
MarkerSeek score 62.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1242
variable_sites
40
pi
0.00763
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.95
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCGGTCTTACAAACTC ACATTTCAAAGAAGGCGGGA 50.0 45.0 57.9 57.7 1360-1497 bp 1.0 83.4
trnK-UUU-rps16_p2 GTCGCGGTCTTACAAACTCT ACATTTCAAAGAAGGCGGGA 50.0 45.0 57.9 57.7 1359-1496 bp 1.0 83.4
trnK-UUU-rps16_p3 GTCGCGGTCTTACAAACTCT TCAAAGAAGGCGGGAGTTTT 50.0 45.0 57.9 57.6 1354-1491 bp 1.0 82.9
trnK-UUU-rps16_p4 GTCGCGGTCTTACAAACTCT TTCAAAGAAGGCGGGAGTTT 50.0 45.0 57.9 57.6 1355-1492 bp 1.0 82.9
trnK-UUU-rps16_p5 GTCGCGGTCTTACAAACTCT TTTCAAAGAAGGCGGGAGTT 50.0 45.0 57.9 57.6 1356-1493 bp 1.0 82.9