Hotspot

ycf1

SSC · 121585-127104 · 5520 bp

Back to Betonica

Pi 0.0249

MarkerSeek score 56.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5520
variable_sites: 137
pi: 0.0249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCATGTGAAACTTGGCTGCT	GAAATGGAAGGGGGCTTTGA	45.0	50.0	58.0	58.1	522 bp	1.0	82.8
ycf1_p2	TCATGTGAAACTTGGCTGCT	GGAAGGGGGCTTTGAATCAA	45.0	50.0	58.0	58.1	517 bp	1.0	82.8
ycf1_p3	GTCATGTGAAACTTGGCTGC	GAAATGGAAGGGGGCTTTGA	50.0	50.0	57.9	58.1	523 bp	1.0	82.4
ycf1_p4	GTCATGTGAAACTTGGCTGC	GGAAGGGGGCTTTGAATCAA	50.0	50.0	57.9	58.1	518 bp	1.0	82.4
ycf1_p5	TCATGTGAAACTTGGCTGCT	AGTCGATATTGAGGCCTGGA	45.0	50.0	58.0	57.9	1065 bp	1.0	82.1