Hotspot

psbT-psbN

LSC · 73123-73195 · 73 bp

Back to Betonica

Pi 0.0548

MarkerSeek score 70.90

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 73
variable_sites: 4
pi: 0.0548
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	47.6	55.0	57.6	57.7	181 bp	1.0	68.3
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	47.6	55.0	57.6	58.4	133 bp	1.0	67.8
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	GTTTTACCGGGTACGCCTTA	47.6	50.0	57.6	57.6	189 bp	1.0	67.5
psbT-psbN_p4	CGAGAACCACCTAAAGTTCCA	TAAGTTTTACCGGGTACGCC	47.6	50.0	57.6	57.6	192 bp	1.0	67.5
psbT-psbN_p5	CGAGAACCACCTAAAGTTCCA	CGGGTACGCCTTATATACCG	47.6	55.0	57.6	57.5	182 bp	1.0	66.2