Hotspot

petA-psbJ

LSC · 62266-63325 · 1060 bp

Back to Betonica

Pi 0.0265

MarkerSeek score 66.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1060
variable_sites: 28
pi: 0.0265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1117-1118 bp	1.0	76.9
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1119-1120 bp	1.0	76.9
petA-psbJ_p3	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	1116-1117 bp	1.0	76.5
petA-psbJ_p4	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	1118-1119 bp	1.0	76.5
petA-psbJ_p5	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.7	57.5	1116-1117 bp	1.0	76.1