Hotspot

trnT-UGU-trnL-UAA

LSC · 45978-46667 · 690 bp

Back to Betonica

Pi 0.0174

MarkerSeek score 65.70

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 12
pi: 0.0174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	GGTTTCCCACACTAAGGCTC	55.0	55.0	57.9	58.2	791-795 bp	1.0	78.8
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	752-756 bp	1.0	78.3
trnT-UGU-trnL-UAA_p3	TAGGAAAAAGCCGGCTATCG	GGTTTCCCACACTAAGGCTC	50.0	55.0	57.8	58.2	842-846 bp	1.0	77.9
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	GGTTTCCCACACTAAGGCTC	50.0	55.0	57.8	58.2	823-827 bp	1.0	77.9
trnT-UGU-trnL-UAA_p5	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	803-807 bp	1.0	77.4