Hotspot

trnG-GCC-trnM-CAU

LSC · 36230-36373 · 144 bp

Back to Betonica

Pi 0.035

MarkerSeek score 73.20

Primer available yes

Label trnG-GCC-trnM-CAU

Feature Metadata

label_name: trnG-GCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 144
variable_sites: 5
pi: 0.035
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnM-CAU_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	211-212 bp	1.0	81.5
trnG-GCC-trnM-CAU_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	212-213 bp	1.0	81.5
trnG-GCC-trnM-CAU_p3	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	247-248 bp	1.0	81.3
trnG-GCC-trnM-CAU_p4	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	248-249 bp	1.0	81.3
trnG-GCC-trnM-CAU_p5	ATGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.9	58.0	288-289 bp	1.0	80.7