Hotspot

trnD-GUC-trnY-GUA

LSC · 30452-30564 · 113 bp

Back to Betonica

Pi 0.0364

MarkerSeek score 66.60

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 113
variable_sites: 4
pi: 0.0364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GGTGCTCTAACCGATTGAACT	TGGCAATATGTCTACGCTGG	47.6	50.0	58.0	57.8	182-184 bp	1.0	72.9
trnD-GUC-trnY-GUA_p2	GGTGCTCTAACCGATTGAACT	GGCAATATGTCTACGCTGGT	47.6	50.0	58.0	57.8	181-183 bp	1.0	72.9
trnD-GUC-trnY-GUA_p3	GGTGCTCTAACCGATTGAACT	GGGGACGGACTGTAAATTCG	47.6	55.0	58.0	58.4	203-205 bp	1.0	71.7
trnD-GUC-trnY-GUA_p4	GGTGCTCTAACCGATTGAACT	GGGACGGACTGTAAATTCGT	47.6	50.0	58.0	57.6	202-204 bp	1.0	71.5
trnD-GUC-trnY-GUA_p5	GGTGCTCTAACCGATTGAACT	GATTCTTCCTGGGTCGATGC	47.6	55.0	58.0	58.4	236-238 bp	1.0	70.9