Hotspot

petN-psbM

LSC · 28974-29749 · 776 bp

Back to Betonica

Pi 0.0219

MarkerSeek score 68.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 776
variable_sites: 17
pi: 0.0219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TCCTACCGCCTTTCTGCTTA	50.0	50.0	58.0	58.4	862-863 bp	1.0	78.0
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TCCTACCGCCTTTCTGCTTA	50.0	50.0	58.0	58.4	860-861 bp	1.0	78.0
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	TCCTACCGCCTTTCTGCTTA	55.0	50.0	57.9	58.4	859-860 bp	1.0	77.5
petN-psbM_p4	ACTCGTAGTGTGGGGAAGAA	CCTACCGCCTTTCTGCTTAT	50.0	50.0	58.0	57.4	861-862 bp	1.0	76.6
petN-psbM_p5	TCGTAGTGTGGGGAAGAAGT	CCTACCGCCTTTCTGCTTAT	50.0	50.0	58.0	57.4	859-860 bp	1.0	76.6