Hotspot

trnS-GCU-trnG-UCC

LSC · 8386-9113 · 728 bp

Back to Betonica

Pi 0.0223
MarkerSeek score 60.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
728
variable_sites
16
pi
0.0223
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 786-797 bp 1.0 54.5
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 785-796 bp 1.0 54.5
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA ACGAATCACACTTTTACCACT 50.0 38.1 58.2 55.5 840-851 bp 1.0 53.2
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA AACGAATCACACTTTTACCACT 50.0 36.4 58.0 56.2 787-798 bp 1.0 52.6
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC AACGAATCACACTTTTACCACT 50.0 36.4 58.0 56.2 786-797 bp 1.0 52.6