Hotspot

ycf1

SSC · 130571-136123 · 5553 bp

Pi 0.0177

MarkerSeek score 49.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5553
variable_sites: 147
pi: 0.0177
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGAATCGTCCAACGCGATAT	CTTTGAACACGCATGGAACC	50.0	50.0	58.2	57.9	1051-1054 bp	1.0	80.6
ycf1_p2	GGAATCGTCCAACGCGATAT	CTGGTTGCTCCGATGTATCC	50.0	55.0	58.2	58.1	972-975 bp	1.0	80.1
ycf1_p3	GGAATCGTCCAACGCGATAT	ATTAGGGGCCGGATTATCCA	50.0	50.0	58.2	57.9	947 bp	0.333333	51.1
ycf1_p4	GGAATCGTCCAACGCGATAT	AATTTGATGCTCCGTCGACA	50.0	45.0	58.2	57.9	875 bp	0.333333	50.8
ycf1_p5	GGAATCGTCCAACGCGATAT	GGGCCGGATTATCCAGATTC	50.0	55.0	58.2	57.9	942 bp	0.333333	50.6