Pi
0.0121
MarkerSeek score
66.30
Primer available
yes
Label
rpl16
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rpl16_p1 | GCACAAATAAATGCAAAGGGTG | TTTTCCACCCAATGAATTCT | 40.9 | 35.0 | 57.3 | 52.9 | 1634-1638 bp | 1.0 | 42.3 |
| rpl16_p2 | GCACAAATAAATGCAAAGGGT | TTTTCCACCCAATGAATTCT | 38.1 | 35.0 | 55.9 | 52.9 | 1634-1638 bp | 1.0 | 42.3 |
| rpl16_p3 | GCACAAATAAATGCAAAGGGTG | TTTTTCCACCCAATGAATTCT | 40.9 | 33.3 | 57.3 | 53.8 | 1635-1639 bp | 1.0 | 42.2 |
| rpl16_p4 | GCACAAATAAATGCAAAGGGT | TTTTTCCACCCAATGAATTCT | 38.1 | 33.3 | 55.9 | 53.8 | 1635-1639 bp | 1.0 | 42.2 |
| rpl16_p5 | GCACAAATAAATGCAAAGGGTG | TTTTTCCACCCAATGAATTC | 40.9 | 35.0 | 57.3 | 52.1 | 1635-1639 bp | 1.0 | 42.2 |