Hotspot

trnL-UAG-rpl32

SSC · 145725-146722 · 998 bp

Back to Beta

Pi 0.0162
MarkerSeek score 47.00
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
998
variable_sites
23
pi
0.0162
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 CTCGCACTGCTTCCTAAGAG AAAAAGGGGTATTGGGAGGC 55.0 50.0 58.1 57.8 1108-1150 bp 1.0 82.9
trnL-UAG-rpl32_p2 CTCTCGCACTGCTTCCTAAG AAAAAGGGGTATTGGGAGGC 55.0 50.0 58.1 57.8 1110-1152 bp 1.0 82.9
trnL-UAG-rpl32_p3 ATCCGCATAAAAGAGCTGCA AAAAAGGGGTATTGGGAGGC 45.0 50.0 58.0 57.8 1742-1790 bp 1.0 81.8
trnL-UAG-rpl32_p4 TCCGCATAAAAGAGCTGCAT AAAAAGGGGTATTGGGAGGC 45.0 50.0 58.0 57.8 1741-1789 bp 1.0 81.8
trnL-UAG-rpl32_p5 GTTAAGAGGCCTGACGTAGC AAAAAGGGGTATTGGGAGGC 55.0 50.0 58.1 57.8 1686 bp 0.333333 50.7